Bacteriostatic water

Reconstitution solvent · Also known as BAC water, sterile water with benzyl alcohol

What is Bacteriostatic water?

Sterile water containing 0.9% benzyl alcohol used to reconstitute lyophilized (freeze-dried) peptides. The benzyl alcohol acts as a preservative, allowing the solution to be used multiple times over several weeks.

Bacteriostatic water is the standard solvent for reconstituting research peptides. Unlike plain sterile water, it contains a small amount of benzyl alcohol (typically 0.9%) that inhibits bacterial growth after the vial is punctured. This makes it safe to draw from the same vial multiple times over 3-4 weeks when stored in the refrigerator.

Key takeaway: Always use bacteriostatic water (not plain sterile water) when reconstituting peptides for multi-dose use. The benzyl alcohol preservative is what keeps the solution safe between doses.

Benefits & evidence

How it works

Benzyl alcohol disrupts bacterial cell membranes, preventing microorganisms from growing in the solution after the vial seal is punctured. This is critical for peptide reconstitution because most protocols call for drawing multiple doses from a single vial over days or weeks.

Plain sterile water lacks this preservative and can only be used once. Sodium chloride (saline) is occasionally used as an alternative but is less common for peptide reconstitution.

Dosing information

Typical dosing protocol
Starting dose

1-2 mL per vial

Varies by peptide concentration
Maintenance dose

1-2 mL per vial

Varies by peptide concentration

The amount of water you add determines the concentration. More water means a lower concentration and easier dosing measurements. Use our reconstitution calculator to determine the right amount.