Follistatin 344

Myostatin inhibitor / activin-binding protein · Also known as FS-344, FST-344, Follistatin

What is follistatin 344?

A recombinant form of follistatin, a naturally occurring glycoprotein that binds and neutralizes myostatin, the hormone that limits muscle growth. By blocking myostatin signaling, it allows muscles to grow beyond their normal genetic limits.

Follistatin gained attention after dramatic muscle-growth results in animal studies, including doubled muscle mass in mice and significant gains in primates via gene therapy. A Phase 1/2a gene therapy trial in Becker muscular dystrophy patients showed improved walking distance and reduced fibrosis. The injectable peptide form has very limited clinical data and a short half-life of roughly 90 minutes. WADA banned follistatin-related substances in 2019.

Key takeaway: Follistatin 344 produces dramatic muscle growth in animal gene therapy studies, but injectable peptide evidence in humans is extremely limited and most clinical data comes from gene therapy delivery methods.

Benefits & evidence

Muscle growth Moderate confidence
Strength gains Moderate confidence
Reduced muscle wasting (gene therapy) Moderate confidence
Fat reduction Preliminary confidence

How it works

Follistatin works primarily by binding to myostatin (GDF-8) and other TGF-beta superfamily members like activin A, preventing them from reaching their receptors on muscle cells. Myostatin normally acts as a brake on muscle growth, so when follistatin neutralizes it, the brake is released and muscle fibers can proliferate and enlarge beyond normal capacity.

The 344 isoform was specifically chosen for research because it has lower affinity for heparin sulfate proteoglycans compared to the 317 isoform, which means it circulates more freely rather than binding to cell surfaces. This also means it has less impact on reproductive hormones like FSH. However, the injectable peptide has a half-life of about 90 minutes, which is why most clinical research uses AAV gene therapy vectors to provide sustained expression over months.

Dosing information

Typical dosing protocol
Starting dose

No clinically validated injectable dose

N/A
Maintenance dose

No clinically validated injectable dose

N/A

Human clinical data exists only for AAV gene therapy delivery (intramuscular injection of viral vector), not for injectable peptide. The Phase 1/2a BMD trial used AAV1.CMV.FS344 at escalating doses via intramuscular injection. Peptide vendors commonly suggest 100 mcg/day for 10-30 day cycles, but this is not supported by published clinical trials. A 2020 case series documented vision impairment in bodybuilders using roughly 1 mg doses (10x typical suggestions).

Side effects

Most side effects tend to improve as your body adjusts.

Injection site pain Common
Flu-like symptoms Moderate
Potential reproductive hormone disruption Moderate
Vision impairment (reported at high doses) Rare
Tendon/connective tissue risk with rapid muscle growth Moderate

Research (10 studies)

Assessing Aβ-independent effects of Module 42 on immune function in vitro. Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026